Cell or tissue lysates are spun through gdna eliminator spin columns to remove genomic dna. The rneasy plus mini kit integrates fast, convenient purification of up to 100 g rna with effective elimination of genomic dna. I have done lots of mini preps before but this is my first midi prep. Plasmid dna purification using the qiaprep 8 miniprep kit 28 plasmid dna purification using the qiaprep 8 turbo miniprep kit 31. Plasmids and cosmids up to 50 kb in size can be purified using hispeed plasmid kit. The qiaamp viral rna mini kit simplifies purification of viral rna from cellfree body fluids with fast spincolumn or vacuum procedures. Can anyone help with plasmid extraction of staphylococcus aureus using qiagen kit fail. Sample preparation using the qiacube follows the same steps as the manual procedure i. Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. Plasmid purification mini kit protocol using a microcentrifuge please read important notes on pages 4 before starting. Viral rna binds specifically to the qiaamp silica membrane, and pure viral rna is eluted in either water or a buffer provided with the kit. Under these conditions, if no expiration date is mentioned on the kit label, qiaprep miniprep kits can be. Qiaprep miniprep kits use silicagelmembrane technology to eliminate the cumbersome steps associated with loose resins or slurries. I have used mini preps on the exact same plasmid with no problem.